Efficient Second Strand Cleavage during Holliday Junction Resolution by RuvC Requires Both Increased Junction Flexibility and an Exposed 5′ Phosphate
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Efficient Second Strand Cleavage during Holliday Junction Resolution by RuvC Requires Both Increased Junction Flexibility and an Exposed 5′ Phosphate
BACKGROUND Holliday junction (HJ) resolution is a critical step during homologous recombination. In Escherichia coli this job is performed by a member of the RNase H/Integrase superfamily called RuvC, whereas in Schizosaccharomyces pombe it has been attributed to the XPF family member Mus81-Eme1. HJ resolution is achieved through the sequential cleavage of two strands of like polarity at or clo...
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Holliday junction (HJ) resolvases are structure-specific endonucleases that cleave four-way DNA junctions (HJs) generated during DNA recombination and repair. Bacterial RuvC, a prototypical HJ resolvase, functions as homodimer and nicks DNA strands precisely across the junction point. To gain insights into the mechanisms underlying symmetrical strand cleavages by RuvC, we performed crystallogra...
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ژورنال
عنوان ژورنال: PLoS ONE
سال: 2009
ISSN: 1932-6203
DOI: 10.1371/journal.pone.0005347